LOCUS       EY193719                 300 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193719
VERSION     EY193719.1  GI:159496013
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 788 was extended to value 788 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 492
            PolyA tail found inside read...SR being truncated to 452
            Potential full insert read...vec-to-vec length: 372
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgagggg cgacaaatgc gtttatgatt tgaaagcact gattttcgcg aactggggcg
       61 tggtggataa gcgtcagaac ttgtactacc agaagccggg cacccagaag cccactcgtc
      121 tgcttgacgc acactcgctt gctgaactga tccaagggac aggggagact acttttgaac
      181 tccgcattac aaaggcttga atgcgatgaa acgggactca gttttcaata aatctcagtc
      241 tatggattta ttcatttgtt gtgtttggac gggagttgaa aaataaatgt ttgttgctta
//