LOCUS EY193714 352 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193714 VERSION EY193714.1 GI:159496008 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 352) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 842 was extended to value 843 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 543 PolyA tail found inside read...SR being truncated to 503 Potential full insert read...vec-to-vec length: 424 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..352 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac atgggctaca tgtccgacgg cacaacaatg ccctggttgt ggtggtctga 61 ctgcaacacc ggctaccaga actacaccaa ctacgtgggc ttcaactatt ggcagtcccc 121 gcaaatgcac cgctaccagt gctcggtgag cgccatcggc ggtgtgagcg gagtcagcgg 181 caactacgac ttcttctgag gagtggggac cagcagaagc gagcaattcg aatgggagac 241 agaattccgt gattttttga gaatccgagt gaacaaatcg ccgtggtcaa tcaatcttct 301 ttacaaaagt gcctagactt ttgattgatc cgtcgtaaac tgttgctgaa ca //