LOCUS       EY193707                 375 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97e12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193707
VERSION     EY193707.1  GI:159496001
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 808 was extended to value 809 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 570
            PolyA tail found inside read...SR being truncated to 528
            Potential full insert read...vec-to-vec length: 447
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca gatgctgagg cagcgttggc acagactttc cggcagcgac gtgagccaag
       61 tgccctgctg agccgatacg gacgtgcagc agtgctgtcg cgttacggga agaggagcga
      121 gcccgagaag gaagaggatg gaaggaacaa acgcgaggaa gaaggcccct ggttcctgtg
      181 caagcgcact cccgacggcc atttcctgcg ctgccgaagt ccgttccagg gctgaacgag
      241 agaaaggcgc gacaatgaaa aaggcgatcc ccggaaaggc taagcatttt catttcaaac
      301 gtggacactc cgtttccttt ccgttctccg tttttatcat caattgtgtc tgtcctttgc
      361 gggaatcggg aatca
//