LOCUS       EY193702                 441 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741830.1| Putative protein family member, with 4
            coiled coil-4 domains, of ancient origin (64.6 kD) [Caenorhabditis
            elegans] gb|AAM69116.1|U41543_7 Hypothetical protein F46H5.7b
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY193702
VERSION     EY193702.1  GI:159495996
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 861 was extended to value 861 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 636
            PolyA tail found inside read...SR being truncated to 592
            Potential full insert read...vec-to-vec length: 513
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga agatctggcg aattggacgt tttgagggca ctggtcagtg acggaacttt
       61 cctgatccgg aaacaaccac acgccgacag acacaagtgg aaacagctca caatagaaaa
      121 cgccctgagc gggggagaag cgactctaag aaggtgctgg ttcgaaaccg agacagatgg
      181 caccaatgaa gtctttttga tcggcagttt cgtgaactgg gagtgcgcct tacgctgcga
      241 gcctgtcccc gatcgagcgt cgacaaaagg catttgggtg gacttgccgc gtggacgaca
      301 cgaattccga tttctgcgtg atggacaatg gcacacggag aatatttatt cggattgttg
      361 gaaccaacac gggaagaaga acaattggct caatgtggaa tgacaattga tgttttaaaa
      421 taataaagaa tatttcgtgc a
//