LOCUS       EY193699                 529 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97e03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_004078.1| discs, large (Drosophila) homolog 1
            [Homo sapiens] pir|I38756 homolog of Drosophila discs large
            protein, isoform 2 - human gb|AAA50598.1| homolog of Drosophila
            discs large protein, isoform 2, mRNA sequence.
ACCESSION   EY193699
VERSION     EY193699.1  GI:159495993
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 854 was extended to value 855 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 863
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtcg ggaagacatt gcattctgga cgtgtcgggc aacgccatca gacgattgca
       61 gaacacagcg cacatctacc caatctccat tttcatcaaa ccgctaaact atcaccagct
      121 gagggagtgg gatcagcaac ttgacgaaga aggcgctttg aagcagttcg agcgttgcca
      181 gcgccaagag cagagtttcg gcgacttatt ctcaacgatt gtgactggcc actcgccaga
      241 tgacgtgttc gcccgagtcc tacacaccat caacgagcaa tcgcgcaccg aaatttgggt
      301 gcccactaac gagccattgc cctgaatatt gtgtgcccac ccagagttgt taaagcttgg
      361 caatttcact tatgcaagaa gggaatgtgc ggcagctgaa atcgatcagt tatatgtatc
      421 cactgttatc catttgtgtt caatccagag aatccactaa aaaaaccttc ttctggccaa
      481 tttctattgt cattttgctt ctcatcccca aacttccgtc ccaaactgt
//