LOCUS EY193698 311 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193698 VERSION EY193698.1 GI:159497139 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 311) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 792 was extended to value 793 (,) putative full insert read: vector-to-vector length is 405 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 553 PolyA tail found inside read...SR being truncated to 462 Potential full insert read...vec-to-vec length: 383 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..311 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggatca aggaatatgt gccaaccaga gaaaaaatga gagctatcgg caagaaagaa 61 gaagatataa gagatatgga ggcagtggtg gacgacgtgc ataaaaaaga atatttgcga 121 ttacagaaac gactagagga tatgaaaaag ctcgacgagg aggtgaagag ggacaaggac 181 ggcgatcgaa aatagagatt cgatctcact tatccaattc atttggcttc ttgaaaatat 241 aggttattgt ttttactttt tagtttttac cattgccttt attgtttctc aattttgaac 301 attgagtgat a //