LOCUS EY193687 394 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97d01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193687 VERSION EY193687.1 GI:159497100 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 394) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 820 was extended to value 820 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 588 PolyA tail found inside read...SR being truncated to 545 Potential full insert read...vec-to-vec length: 466 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..394 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggccg caaacggctc attacaccag cagtaatcta tttgaccttg ataacctact 61 tggataactg tggtaattct agagctaata catgcacaaa aactcccaac gcaaggaggg 121 agcgcatttg ttcgccacaa aaccaagccc cctcgggggt tgagtgttga atcagagcaa 181 ctaagctgag cgcacggtct tgcaccggcg ccgtatcatt cgagcttctg ccctatcaac 241 tttcgacggt agtgtctctg gctaccgtgg tggtgacggg taacggagga tcagggttcg 301 actccggaga aggggcctga gaaatggcca ctatgtctaa ggatagcagc aggcgcgcaa 361 attacccact ctcagcatga ggaggtagtg acga //