LOCUS       EY193674                 400 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193674
VERSION     EY193674.1  GI:159497058
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 845 was extended to value 846 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 594
            PolyA tail found inside read...SR being truncated to 550
            Potential full insert read...vec-to-vec length: 472
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaga actgaccaga cggggcattc tcaccggttc cagcatcgcc atgcaagcgc
       61 tcggcatcgc aacagtgatc acagtgtccg gctatttgac gttcgtgatc ctcgtctcgg
      121 aaatgctcgg cgttgacagc tggaagcagt tcggccgcaa aatgacaaac atgttcggcg
      181 accgcttccg gatagcaaag gacgggggag agaactatgc atcgctcaca gaactgtttg
      241 aagctgccga aaggcaacaa aggaagaagt gatctcattg ctcaaactca tattcaagtg
      301 catcaacatt cagcaggcag tcgtctccct tcaatttctt aggctcagat caaacatcac
      361 aagatccatt gtttctactt tgttctttgt ttgcaccaca
//