LOCUS EY193673 318 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193673 VERSION EY193673.1 GI:159497057 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 318) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 839 was extended to value 840 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 509 PolyA tail found inside read...SR being truncated to 468 Potential full insert read...vec-to-vec length: 390 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..318 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccagggcg cgagatttgt tcgtacccaa agaagtacat gcactcgtgt cccagtgagc 61 tggccaagcg cttcgaggag aagggactcg ttgtcgccga agatcccgag ttcacgttcc 121 cgatggaccc ggagggggca aaacccaaga aggagcacat ctactcctcc gccatcgagc 181 gctttgtgct ccgcccatga gccgaacgcg cagcgcagaa ttgtaggata aagtgaacgg 241 actgtttcta gccgctgaga agaccttttt ctctttgtgt ttctgctaac ttcacgtgaa 301 ataaaggtat tttaggca //