LOCUS EY193670 311 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97b06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAG44365.1| cathepsin B-like cysteine protease [Leishmania donovani], mRNA sequence. ACCESSION EY193670 VERSION EY193670.1 GI:159497054 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 311) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 755 was extended to value 759 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 504 Potential full insert read...vec-to-vec length: 310 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..311 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc agttgctggg ccgtgtcctc tgcctccgtt gctggggcgt ggacggcccg 61 accaaggtgc cctactggct ggtggccaac tcgtggaaca ccgactgggg agaggacggc 121 tacttccgca tccgccgtgg caccaacgaa gcctacatcg agagctgggg cgtcaactac 181 gcgtcagcga tcgttgagtg aagtctgaag tgggagagga aatattggtc acctcaattt 241 tatgatggca cacattgaac agttatggaa tagcacaatc tattatgaag atggataaaa 301 aaccaattgc a //