LOCUS EY193669 226 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193669 VERSION EY193669.1 GI:159497053 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 226) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 836 was extended to value 837 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 421 PolyA tail found inside read...SR being truncated to 378 Potential full insert read...vec-to-vec length: 298 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..226 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga cgaagaggtc ttcaacgggc agacaggctg ggactcgctt ctgcggaact 61 cgcgcggatt gttggacatc tactgctgat gacgtgatga ctgacaatgg tggcattcgg 121 actgtgaaga gggactccaa ttttatttat tcatcggatt cctgtatatt tggctttttg 181 tgaacaatct atttacggaa taaagaataa atcacaccaa ggcaga //