LOCUS EY193668 192 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa97b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193668 VERSION EY193668.1 GI:159497052 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 192) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 844 was extended to value 844 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 435 PolyA tail found inside read...SR being truncated to 343 Potential full insert read...vec-to-vec length: 264 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..192 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg gaaaacagca ggtgaatcaa gttcaagtga tgatttcacc cgcacaagca 61 gcgaggagga gatgcgccaa agcaccgagg atgagataaa tgaggaaaat gatgacgacg 121 acgagcttta gagtctgttc agactttgca ataattgttg ctcttcatca gttttgaaaa 181 aagttgaaac ta //