LOCUS       EY193661                 553 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa97a07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193661
VERSION     EY193661.1  GI:159497019
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 818 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 744
            PolyA tail found inside read...SR being truncated to 704
            Potential full insert read...vec-to-vec length: 625
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc caaattgaat aattgggttc gaagttttaa gtaattccgc gccaaataca
       61 ttttaatata atacttattt tgaactgaat ttgacctcaa aatgagtgaa agcggcgaag
      121 gaagcatcag caagcgggag aagaacaaat tggcacagac aaagcacaga gaggtaaaag
      181 tgttttggtc ttgattattt ctatatttct ttacttcccg caaagcagtt gttgcaaatg
      241 agcaaaaata aaatggaaca gtcatctgcc gacggtccgc gactatgcga tgtgtacccg
      301 agcaggatac gtccactggt cgcgaatcga acagcgatcc tgatagttga tgtgcagaac
      361 ctggtgacgg acgagggcag gtatccgctc agtccagagt tttacaaaga ggcgaaagag
      421 aatgtgatcc caaatatccg aaagctgatc gacaccgcgc gtaattataa cgtcgaggtg
      481 gggattggtt tttattttat ttttactagt ttttgaaaaa tacatactaa ccaaaaatac
      541 ctagtagggc cga
//