LOCUS EY193647 134 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa96g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193647 VERSION EY193647.1 GI:159496946 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 134) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 235 (,) original QR value of 718 was extended to value 722 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 383 PolyA tail found inside read...SR being truncated to 284 Potential full insert read...vec-to-vec length: 206 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..134 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggttga ttcatctgga gtgattattc gccggaaagg cagtgtttag gatttttaca 61 atcacatccg tcccaattcg ttcgcattat gtgtacttta cttttttttg tttttgtgta 121 aatctctagc agca //