LOCUS EY193642 420 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa96g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193642 VERSION EY193642.1 GI:159496941 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 420) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 614 PolyA tail found inside read...SR being truncated to 571 Potential full insert read...vec-to-vec length: 492 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..420 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac tttcccattt ccgaagccaa ttcaccacca cactaccttt tggccaattt 61 gtccaaaatg cagatgaacc acttctttct tgtttcccta ttcgctttgt gcacggccag 121 cagaagcaga gtgagtgcag cgggcagtgc cgaaatgatg gatggatctt caggagctgt 181 gctcgatgag gagattgttg ttccctgaaa aaagttgtac cgtccgagag gtcctccccg 241 ctttgggaaa cgggcactgg tgctgatgga cgacgagtct gtgccggtgg cttggaggaa 301 gggaactgaa tgatggaaga gcggcaggaa agggcgcatg cgcagattgg tccattttca 361 atcgaactga cgaatgttgg tgataaatga taatgtgtgg gcgaataaac tccactctga //