LOCUS       EY193638                 566 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa96f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497174.1| Ubiquitin conjugating enzyme UBC-20
            (22.3 kD) (ubc-20) [Caenorhabditis elegans] pir|T33629 hypothetical
            protein F40G9.3 - Caenorhabditis elegans gb|AAC68796.1| Ubiquitin
            conjugating enzyme protein 20 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY193638
VERSION     EY193638.1  GI:159496937
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 827
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtat caaacctagg tattttgtat gtgcgtcatt tcaaaagcat caaagtgatg
       61 tcaaacatag gatatgtaag gatccagaaa gagtgcaaag aaatcattct ttgtaaagag
      121 ctccaggaaa atggaattag ccttgtgcct aataatgaag ctctatcaga actggacgga
      181 agaattaaag gacctcccga cagtccatac gaagatggga tatttaatct ggaaatcaga
      241 atcccgcacg aatatccatt taaacctccc aaagtgaaat ttacgacacg aatatggcat
      301 ccaaatatca gttcgcaaac gggcgcaatt tgtttagata ttcttaagga tcaatgggca
      361 gcttccatga cacttcggac agtccttctt agcgttcagt ccttacttgc aacgccagag
      421 ccaaaggatc ctcaggattc cacagcaaaa tattgggctc agcattatgc gaacgctcct
      481 ggagaaatga atacggatat gctcaaaagt gtgaccaagt tgagagaaat gggcgcagct
      541 gtggatgtgt gtatttctgc gttgag
//