LOCUS EY193634 412 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa96f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193634 VERSION EY193634.1 GI:159496904 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 412) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 860 was extended to value 864 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 605 PolyA tail found inside read...SR being truncated to 565 Potential full insert read...vec-to-vec length: 484 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..412 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgg gtggcggcgg ccaagaaaga gtctctgcgc ttggcacaaa tcggtgggca 61 tgtctcacag tttcagtcag tgcgaaacgt acagccacca ccgcaggagt ccacatcgtc 121 cggttcaaag cgaacggcga cggcggccgg gattggatcg cgagctggat ctggttctcc 181 aaaaaggacc gccaatcgag tggcatgcga cctctgtgaa tgtcgttttc cagcggtggg 241 cgacatgtac gagcacatgt ggagttacat ccagtgcacg caaagaaaat gaacctcccg 301 aagatatgat gagaggagag gccagcagaa aatgttccat tcactcgcga tagatcactt 361 tctgggttgt tacaaatgtt aatttccgtt aaataaaaat gttgtaaccg ca //