LOCUS       EY193624                 408 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa96e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_220532.1| DNA-DIRECTED RNA POLYMERASE BETA PRIME
            CHAIN (rpoC) [Rickettsia prowazekii] sp|Q9ZE20|RPOC_RICPR
            DNA-directed RNA polymerase beta' chain (Transcriptase beta' chain)
            (RNA polymerase beta' subunit) pir|B71724 dna-directed RNA polyme,
            mRNA sequence.
ACCESSION   EY193624
VERSION     EY193624.1  GI:159496864
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 879 was extended to value 880 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 600
            PolyA tail found inside read...SR being truncated to 559
            Potential full insert read...vec-to-vec length: 480
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ctgtagtgga aggaagtact atagttgcat ctctagaaag tatcgtactt
       61 ggtagagttg ctgcaaccga catacaccat ccaatgacaa aagaattgtt aatcaaagca
      121 ggagaactaa tagatgaaga tatagtaaag cggattaaca ttgctggagt tgatgctatt
      181 aaaattaggt cacctttaat gtgtaaggta agtccaagtg tatgttcttt atgttatgga
      241 cgagatcttg caactggtaa gattgtatca ataggtgagg cagttggtgt gatagctgca
      301 cagtctgtag gagaacctgg tacacagtta actatgcgta ctttccacat tggaggagta
      361 gtaactaggg gtatagactc atctaacgtt atggcttcaa ttaacgca
//