LOCUS       EY193543                 498 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_506448.1| Predicted CDS, estradiol 17
            beta-dehydrogenase family member [Caenorhabditis elegans]
            sp|Q17704|DHBW_CAEEL PUTATIVE STEROID DEHYDROGENASE C06B3.5
            pir|T18981 hypothetical protein C06B3.5 - Caenorhabditis elegans
            emb|CAB01115.1| Hy, mRNA sequence.
ACCESSION   EY193543
VERSION     EY193543.1  GI:159497458
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 788 was extended to value 789 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 715
            PolyA tail found inside read...SR being truncated to 649
            Potential full insert read...vec-to-vec length: 570
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa catcgcttcc gcggccgcct acctcaactg gtactgggcg tcggtctatt
       61 ccgcgaccaa gagattcatg tgctctcttg ccgctaccct tcggaaagag tacgcagttg
      121 agggttcggg cgtcgtcatt caaaccatct gtcccatgtt ggtggccacc aaattgggcc
      181 gctatgcgga tgcactgtcg ctgtcgatgt ccccttctgc cgaagcattt gtgcgacagg
      241 caattcgcac gattggactt gcggacgaga cggccggctg cgtgtcgcac caaattcagt
      301 cggagttact tttcggctac attcccggat ggattcttga ttggacgatg cgcttcgaag
      361 catcgaaaag acgtgaccga gcactgtgcg aagaagcaaa agagaccaat aaagagaatt
      421 gacaactgag aaacacaaaa attcattttt tcaaataatg cttaattatg tataatttat
      481 aaataagttc tcttcnca
//