LOCUS       EY193540                 324 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193540
VERSION     EY193540.1  GI:159497455
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 846 was extended to value 847 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 517
            PolyA tail found inside read...SR being truncated to 475
            Potential full insert read...vec-to-vec length: 396
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga gagcagcaga aggacgaggc ctaagctccg acatgcgcaa aatgattgct
       61 gaaccttatt cacttgttaa ttgtttatct atttattaat actctttgaa ctttaaaaac
      121 aatccgtgga catgatactc ttctctccag aaaactcatt atcattatac tctgactgac
      181 tcgtgactct cgacgataac tttttgaact tatgatctta ccctgcctat gctggttgtt
      241 tatctctttg gcctggttag ttatatgtta ctcaaaacca acggtttatc tcttcaatga
      301 tcggtataaa atcaaggaat gcga
//