LOCUS       EY193539                 640 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_506513.1| TRNA Methylase TRM-1 (trm-1)
            [Caenorhabditis elegans] sp|Q23270|TRM1_CAEEL
            N(2),N(2)-dimethylguanosine tRNA methyltransferase
            (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
            2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2, mRNA
            sequence.
ACCESSION   EY193539
VERSION     EY193539.1  GI:159497454
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 834
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggccg gcacaagtcc tgccacgaaa ttgccctccg catactgctc cggtcgattg
       61 actcccatgc aaatcgctac acacgctaca tcaccccttt gctctctgtc agcgtcgact
      121 tctacattcg ctgtttcgtc aaagtccgct ccggagccgc gcccgccaaa gacagcgtga
      181 ccaaactcgc ccatgttttc tgctgcgcca cgtgccagag tctggatttc cagcccctcg
      241 tgcgaaaaag cgtcaatggg ccgagcgtca agttctccag cggccacttc cagagcaaac
      301 tgatcagaaa cgacccgtca acaagcgacg gcagaggaag tggggctcag tgtgcacatt
      361 gcggtggaac cgccatccat atgggcggcc ccatctgggt tgcacctatc cacgacgagg
      421 actttgtgca gaagctccgg caaaggttgc gagagacgcc cgcggaacaa cgcctgggaa
      481 cacacgaccg actggagggg gttttggcgg tggtctcgga agagctgcac gacgtgccgc
      541 tctactacga acacgaccag ttgatgaatg ctgtcgagtg ccctgttccc aaaatgtcaa
      601 gttttcgctc ggcactgctc aacgccggct accgctgttt
//