LOCUS       EY193535                 408 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193535
VERSION     EY193535.1  GI:159497418
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 818 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 647
            PolyA tail found inside read...SR being truncated to 558
            Potential full insert read...vec-to-vec length: 480
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa ggtcaacgtg ttgtttcaac ttcgagcact cgcactgtcg tggagggaag
       61 tcaagcgcaa cagcgagaag gcggtgtaaa tattttcgtg gaaattgtgc cgtgggacag
      121 aaggaagctt cgccgatttg atattgccgt acagttaggt gacaccgtcg caatgctcaa
      181 ggaaaagctc gctggacagg aacgaattcc aattgatcaa ctacgtatct ggcggatcgc
      241 agagggtgat gaatacgaat tggcggggga ttggacgaca ctggccgcaa acggcatcaa
      301 aagcgggact caaatccgtg tgcgcaacca cttccgcaaa aaattcgatc agcagtagtg
      361 attgatcgct atgccacgtg ttattgttgg ataaaatgat tttgttta
//