LOCUS       EY193534                 349 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_498863.1| Homology with E.coli lepA protein
            [Caenorhabditis elegans] sp|P34617|YO81_CAEEL Hypothetical
            GTP-binding protein ZK1236.1 in chromosome III gb|AAA28191.2|
            Hypothetical protein ZK1236.1 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY193534
VERSION     EY193534.1  GI:159497417
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 856
            PolyA tail found inside read...SR being truncated to 499
            Potential full insert read...vec-to-vec length: 421
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat tgaaaaaagt aggaatacct cgacaacaat tcgaagtagt gattaaggct
       61 tgtcttggac attcaaaaaa ggcagtcgca caggcgaaaa ttgctccgat gaaaaaggac
      121 tttacccaag tcttaaaggg gaattttgtc gccgatcgtt ttggaaagaa actcaagcac
      181 caaagagagg ggaaagagaa actgaagatg ctgggcactg tccaggtccc aagagaagct
      241 tttttgaaca ttgtgaagaa aggataggaa ataataggat tatttgtgat atttaaaatt
      301 taatctgtct aaatgattgt ttgttaaatt ataaaacgat gaaagaaca
//