LOCUS       EY193522                 181 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95c09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193522
VERSION     EY193522.1  GI:159497371
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 181)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 393 (,)
            original QR value of 708 was extended to value 709 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 542
            PolyA tail found inside read...SR being truncated to 332
            Potential full insert read...vec-to-vec length: 253
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatg ggttgtcctg ttgccgcttt cgaccgtcga attcaatcgg gtcaatggga
       61 acaaccctat ttagatccat cgattggttg gttcacttaa tacccccatt tttgttcaaa
      121 tgtgccaaat tggtccaaat gtttattatt ggcgcattcg ctaatacaca ggggacacac
      181 a
//