LOCUS       EY193517                 372 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_500039.1| Extracellular calcium binding protein
            OSTeonectin precursor homolog 1, Secreted Protein Acidic and Rich
            in Cysteine related; involved in body size and growth rate control
            (30.2 kD) (ost-1) [Caenorhabditis elegans] sp|P34714|SPRC_CAE, mRNA
            sequence.
ACCESSION   EY193517
VERSION     EY193517.1  GI:159497332
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 820 was extended to value 820 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 566
            PolyA tail found inside read...SR being truncated to 523
            Potential full insert read...vec-to-vec length: 444
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gagaaaaagc acgtgtaccc ggtgatctgg aagttctgcg atctggatat
       61 caagccgcac gacaaacacg tctcacacca cgagctgatc ccgataactg cgcccgtcat
      121 cccgatggag tcgtgcatca agccattcct agagggctgc gacaccaaca aagacggcgc
      181 catcacgatc aaggagtggg gcaagtgcct gggactaaag gaaggcgaga tccaagaacg
      241 ctgctagagg ggaacgatga ataaaagtgc caaccgcctt ttcctgagaa acagaaaatg
      301 cccagacaaa ttttgaatcc aatgcttgct taatgcgttt cagctgtcga ctttcgataa
      361 acccaattgt ca
//