LOCUS EY193516 341 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa95c03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193516 VERSION EY193516.1 GI:159497331 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 341) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 858 PolyA tail found inside read...SR being truncated to 493 Potential full insert read...vec-to-vec length: 413 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..341 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggccg acttggcaaa gctgatgaat gagaaggcgg agcctaacgc aaacgtggta 61 gaggtcatcg agaagtacaa acacgtcttt gatcgcttgc cgaatcaggc aaagtcggtt 121 cacgacttca tttttgggaa gaagggagtc aagcggaccc atagcgcagg ggagaaggat 181 gctgctgatc cgaagaaggg caaatgtggc gagccgacta ccaatgaggg ctaattgagg 241 gacgaatgaa ccggaaggag cgaatttcta ggaaatgaca atctaattta tttcgattat 301 cttctctttt taaaacgaca tgcataaaat aaaaggttgg a //