LOCUS EY193511 393 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa95b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAD44625.1| cathepsin B1 isotype 2 [Schistosoma mansoni], mRNA sequence. ACCESSION EY193511 VERSION EY193511.1 GI:159497295 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 393) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 588 PolyA tail found inside read...SR being truncated to 545 Potential full insert read...vec-to-vec length: 465 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..393 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac gacttcatgt tctacaagag cggcgtgtac cagactgtgt tcccgtggcc 61 gctgggcgga cacgcagtgc gcattgtcgg ctggggcgtg gacggcccga ccaaggtgcc 121 atactggctg gtggccaact cgtggaacac cgactgggga gaggacggct acttccgcat 181 ccgccgtggc accgacgagt cctacatcga gagctggggc gtcaactacg cgtcagcgat 241 cgttgagtaa attggactgc aaggggcaag aagattacaa cacaaagcga acatataatt 301 tataacaaac acctttgatc atccatgttt gtttgttcct tttaccagtt tttttacttc 361 gaagtccgat tgaaataaag aagatgcatt cga //