LOCUS       EY193493                 485 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa95a03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193493
VERSION     EY193493.1  GI:159497252
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 800 was extended to value 800 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 682
            PolyA tail found inside read...SR being truncated to 643
            Potential full insert read...vec-to-vec length: 557
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg catcgtggac acgctgaagc cctactcccc gctgaagagt gcgctcgcca
       61 aaacacacag gaaaatatac aaaaagcctc gagtcgtcgc aagtccggcg aactacgctg
      121 cgcgctttct gaagatggtc acgaccaatt attttttccc tgtggaccag aatgggctga
      181 aactgctgaa ctcgcgaaag aggtcggggg gatccgtgcg cagagcttca gcgagactgc
      241 tgaagagtcc gaaattggtg tccccaatca gttgattcgg atttggtggc tctggaaatg
      301 aaactgcatt tctgtctcta gctctgtgat tgttgtgttc aaaaagattg ctgctctcga
      361 gtattgccag cgatcagagg ccgtcggttc gactaccggg gaatccccag accaaattgg
      421 ttcccctttt gatgcttttg attgcgtcca ttatccgcat aaatggcact gaactctgaa
      481 aaaga
//