LOCUS       EY193481                 585 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499279.1| Putative membrane protein, with at
            least 4 transmembrane domains, of eukaryotic origin [Caenorhabditis
            elegans] sp|Q10045|YRT2_CAEEL HYPOTHETICAL 28.6 KD PROTEIN T07A5.2
            IN CHROMOSOME III pir|T24631 hypothetical protein T07A5.2 -, mRNA
            sequence.
ACCESSION   EY193481
VERSION     EY193481.1  GI:159497177
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 820 was extended to value 820 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 827
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaca gaaaaaaaac caaagaccaa tgggctcgag acgatcctgc ttttcttgtg
       61 ctactcgccc tttccctgct cggctccagt gtgctcttcg ctctggcact aaatctatct
      121 ttcgtctctt tttgggcatt cttcctctgg gcagtgttca tcgactgcat cctggtcgga
      181 attgtggtgg caaccaccct ctggttcatt tccaatcgat atttgcgccg agtgaaggac
      241 caagacgtgg aatggggcta ctgcttcgac gtgcacctga acgccttttt cccgcttctg
      301 gtcttgctcc acgtcatttt accgctgctc ttctacggtc tgatcgatta cccgaatttc
      361 ctggctcgcc tgctcgggaa ctccatctgg tgcattgccg tcatctacta catctacatc
      421 accttcctcg gctacacagc gctcccaatt ctgaaaaaca cgcacgtgtt cctctacccg
      481 atcacgttcc tcttcatctt cttcatcgca acgatcagcg cgggatggaa catttcgcag
      541 accgccatgg acttctacca cttccgggca acgagtgcga atcgc
//