LOCUS       EY193476                 495 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193476
VERSION     EY193476.1  GI:159497172
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 822 was extended to value 826 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 686
            PolyA tail found inside read...SR being truncated to 646
            Potential full insert read...vec-to-vec length: 567
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca atagcttccg atgtagcaag tgaggcttac cagcaaatgc agcaattggg
       61 acaaatttac gacagcctga accttttgtt tcgcagatat tcgcacaatt tcccatcctc
      121 cgtgaaatcc ctgcgactgg tttcgatccg cggacatctt cacgctgatc ggaatgttgg
      181 gctgctcaaa gagcacgacc tagcattgct tcgtcgcgaa attgagggaa ttgggcaaga
      241 accggcagac atggaggact tcgcaaaaat cgacgtcctt ttcgcgatga acaatccggc
      301 cttcaggcgc tttacaattt cgaaaggatc caggaaagtg gcggcgggaa gtggaagaag
      361 aaagaaatgg aactagccca attttgtatg ttcgatcgga caagtgtttg gctccgcatt
      421 aaacaaattt ccgcctttat tataccaaca tatgtttaaa aaccgatttg tgttgcaaaa
      481 gtagtgagaa cctga
//