LOCUS       EY193474                 545 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94g01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193474
VERSION     EY193474.1  GI:159496443
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 620 (,)
            original QR value of 808 was extended to value 808 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 770
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga aagacggctc gagttcgagg accggcgaga gtcctgtcta catggctggg
       61 agagtcatga gcactggcgc gggagccaga aaacacgcaa cggcactgag gaatccgatt
      121 gcacccatcg atttggatcg agagcaggtg cggatgctct gggacgcgag gactgacggt
      181 ggagccaaag ccgaatgcac aacacaagga tgacgaatga ggaaagaaaa taagagaaaa
      241 tgttcaattc cttttagtga tcgagtgctg ttccatttct attcccccac tctcacgttg
      301 tctcccgttt cgatcattta tgcgcgggga cgcgcattcg gggagccacg ggaatgttat
      361 gggaacaaag tccatgagta ggagcaaacg tcaatggggg ccatggctag ggccaagccg
      421 ttaagcctcc cataaatcat tgtggcccac caatgctctg gggagaaggc atcttcttta
      481 aagcccttcc cttttctatt cttggggctc actctctata tcgcccaacc atcagccgat
      541 gttac
//