LOCUS       EY193473                 512 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499959.1| Von Willebrand factor type A domain
            containing protein precursor family member (46.6 kD)
            [Caenorhabditis elegans] gb|AAF98615.2| Hypothetical protein
            C18H7.1 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY193473
VERSION     EY193473.1  GI:159496442
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 786 was extended to value 786 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 702
            PolyA tail found inside read...SR being truncated to 663
            Potential full insert read...vec-to-vec length: 584
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa gcaggcggac gtgcacgagg gggcacgtcc gggactggcg aacaaggtga
       61 tgatcgtgtt cacggacggc tggcagaaca agggccccga cccgaacgag gcggccaggg
      121 cagcaatggc agccgggttc cagatgtact ccgtgtcggt tgtgagtcat cgggacgacg
      181 ccattcccgt taatgagccg actctggaag acattgtgcg ggacccacgt ggcgaccact
      241 tcaccgacgt caatttccag tcactcatca acattgtacg cgaacgcaat gtaaagtgct
      301 ttggccatct ctgatttgtg cgaaaaggcg acagtcgagt gatgttggtt ggaatgcagc
      361 tgtttaaacc tttaaactgc acagtgttcg ttcatgcctc tctcttgcca ttctaatatt
      421 ttcgttgtac catatcctaa cgcacaatag agcctatttt tctttcaatc ggtagtgcct
      481 ctcgacaaat taataaattc actcactctc ca
//