LOCUS       EY193467                 500 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94f05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAA92741.2| Hypothetical protein H02I12.8
            [Caenorhabditis elegans] emb|CAB07222.2| Hypothetical protein
            H02I12.8 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY193467
VERSION     EY193467.1  GI:159496405
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 693
            PolyA tail found inside read...SR being truncated to 651
            Potential full insert read...vec-to-vec length: 572
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggccg gaacagtggg cagatccaga agtgttcaat cccgaccgct ttttgcgcga
       61 gaatttgcaa ggacgccatc cattctcgtt tgtgcccttc tccgcgggca gtcgcaattg
      121 catcggacgg cgcttcgcca tcatggagga gaagaccgtg ctcgtctgga tcctgcgcaa
      181 ctttcaaatc aaagcggtga agcgtcttga ccaggtccga cacaaagcgg aaatcatcat
      241 ccgcccgacc accgaaatat cgctcaaact gaccccgaga aacaatgacg aataaagctc
      301 aagacggatt ttattttcct atagatgatg gctagctttt atcatttaag atctaaattc
      361 ataatctaac tagtatgtga ccctcattca cccattcatt catgttgttg accttctaat
      421 caatccatcc atgttcagac attgtgatac tcgaatgata atttctaatg atttcttaaa
      481 tataaatttc tgattctaga
//