LOCUS       EY193459                 357 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAA70693.1| cathepsin L-like cysteine proteinase
            [Heterodera glycines], mRNA sequence.
ACCESSION   EY193459
VERSION     EY193459.1  GI:159496366
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 553
            PolyA tail found inside read...SR being truncated to 508
            Potential full insert read...vec-to-vec length: 429
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ccatggtgtg ctggtggtcg gatacggcac ggactcggaa cagggcgact
       61 actggattgt gaagaacagt tggggaacga agtggggcga gaagggctac atccgcatgg
      121 ctcgcaaccg caacaacaac tgcggcgttg cctcgcacgc ttcctacccg ctggtctgaa
      181 cggtccctcg cttggtttgg gggatgtcca ttgatccgat tcggcagtcc atcgatgcac
      241 ataagattaa gtaaacttgc tttttgtttt agtaacctta aaatcgtatc ttgcaagtca
      301 catatatcca catgcaacaa ccattttgta aattttcaat aaatggcgaa ctttaga
//