LOCUS       EY193454                 350 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193454
VERSION     EY193454.1  GI:159496361
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 822 was extended to value 823 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 544
            PolyA tail found inside read...SR being truncated to 501
            Potential full insert read...vec-to-vec length: 422
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtc cctatgtgct ctatcggacc acacgcgaga ttgccttcaa caactcggtg
       61 gaactgaccg gatacacagc cgagtactgc caggcaccgt tctcgcgtct gaccagcctg
      121 tacatcagtc tgcagttcga tgtcacggcc acgctgcgct actactacgg gcaccaggag
      181 caggtcactc tgtccatgca gcagcaggtg tgctgtgtgc ccagcggaaa ctgcacctcc
      241 ggatgggtgg acctctgaaa tggagaagga agagatttca ctttttgcgc tagtcttggc
      301 atactaatca agcatgactt cggatcaaat gtcagaaaag agattttcga
//