LOCUS       EY193452                 375 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94d12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193452
VERSION     EY193452.1  GI:159496359
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 570
            PolyA tail found inside read...SR being truncated to 527
            Potential full insert read...vec-to-vec length: 447
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg ccaccgaaag ttgacaccac tcctgtggcg tccattttca aagaactcag
       61 accgtccgag aaacgttatt cgctcctgca aaagaccgaa ttgtgatgca aggtgtggaa
      121 ttgtcgaagg gacaaagttg ttttctgtga ttaaaactaa attaattttg gtctactatg
      181 tatgttttgt gagagtatta tttttaaatt gggtattttt ctgaatctaa attttgctct
      241 attgacgcat ctgtcttttc ttgtcagtta gtaattgttt ttacaacaca tttcacttac
      301 aaataataat attccggttc attcacttat tttgttaatg tcgcgctatt acatgataaa
      361 gagggctgaa gtaca
//