LOCUS       EY193451                 394 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94d11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499264.1| Citrate synthase (51.5 kD)
            [Caenorhabditis elegans] sp|P34575|CISY_CAEEL Probable citrate
            synthase, mitochondrial precursor pir|S42370 citrate (si)-synthase
            (EC 4.1.3.7) precursor - Caenorhabditis elegans emb|CAA83004.1|
            Hypothet, mRNA sequence.
ACCESSION   EY193451
VERSION     EY193451.1  GI:159496358
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 663
            PolyA tail found inside read...SR being truncated to 623
            Potential full insert read...vec-to-vec length: 393
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa tgtgtacaag gtgacaccgg acatactgct ggagcagggc aaggcaaaga
       61 acccgtggcc gaacgtggac gcgcactccg gcgtgctgct ccagtacttc ggcatgaagg
      121 agatgaacta ctacactgtg ctgttcggtg tgagtcgtgc gctgggctgc ctctcccaac
      181 tgatctgggc gcgtggaatg ggactgccgc tggagcgccc caaatcgcac agcaccgacg
      241 gactgacgaa actggccaaa gcggcaaagg cggcaaaata gacacatttg gatgaaagca
      301 gattttccct aaaatccctg atagacatca ccttttcacg gttttccatc acattgttat
      361 ttgagagcag catctatgca gactgacatt ttta
//