LOCUS       EY193450                 273 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193450
VERSION     EY193450.1  GI:159496328
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 851 was extended to value 852 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 518
            PolyA tail found inside read...SR being truncated to 424
            Potential full insert read...vec-to-vec length: 345
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gcagagcagc actggattcg gtccgtactg ctgcgaaagc tgctcatcct
       61 tggtgtactt gcccacaaag agactctcct cgcagatggt gcacttgaaa caggcgtcgt
      121 ggactgaaat ttaacaattg aatttctgac ctttgaccaa atctttgtca gaatatcaat
      181 tttccccacc aaagcgtttc tcgttgttct tcactttgcg atccctcact tcaatgtcct
      241 tgttgcaaat ggaacaaatc ggcaaacggt gca
//