LOCUS       EY193449                 588 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_759896.1| Conserved hypothetical protein [Vibrio
            vulnificus CMCP6] ref|NP_759902.1| Conserved hypothetical protein
            [Vibrio vulnificus CMCP6] ref|NP_759940.1| Conserved hypothetical
            protein [Vibrio vulnificus CMCP6] ref|NP_760020.1| Orf122-l, mRNA
            sequence.
ACCESSION   EY193449
VERSION     EY193449.1  GI:159496327
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 824 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 830
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaag gcagcagtat taagctaaat tattcttatt aatccaacct tttaggttgg
       61 attctaaatt tgagagtttg atcctggctc agaatgaacg ctggcggcag gcctaacaca
      121 tgcaagccga acgaggttgt attatagctt gctgtataca acctagtggc agacgggtga
      181 gtaatgtata ggaatctacc tagtagtacg gaataattat tggaaacagt aactaatacc
      241 gtatacgccc tacgggggaa agatttattg ctattagatg agcctatatt agattagcta
      301 gttggtaggg taatggctta ccaaggctat gatctatagc tgatttgaga ggatgatcag
      361 ccacactgga actgagatac ggtccagact cctacgggag gcagcagtgg ggaatattgg
      421 acaatgggcg aaagcctgat ccagccatgc cgcatgagtg aagaaggcct tcgggttgta
      481 aagctctttt agtgaagaag ataatgacgg tattcacaga aaacgtcctg gctaactccg
      541 tgccagcagc cgcggtaata cggagagggc tagcgttatt cggaatta
//