LOCUS       EY193448                 447 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193448
VERSION     EY193448.1  GI:159496326
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 810 was extended to value 811 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 638
            PolyA tail found inside read...SR being truncated to 598
            Potential full insert read...vec-to-vec length: 519
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagg agcttatagt agcacatgtt acttgttttt ttattaaatc cggggatgaa
       61 tgaaagattc aaatttgatg taaattatat ttttagaatt taataaaaat ttaataatta
      121 attttttttt ttaaactaag aaaagtcttt ggaaatttat tttttataaa tattataaaa
      181 attttttttg gaataagatt taataaaaaa attaaacttt gaaatattaa ttaaaatttc
      241 ttcagagtta acaggatttg aatattatta tttaatattt ttttactttt acatcgatgt
      301 tgtttttaat ttaattatat taaaagaaga ttatatttaa aaaggactgt tcttctatta
      361 tgattaacct gaaattagtt taattcgtcg taaggtagag ttgtttatct agtttataaa
      421 ttttaaaaat aatttcctgt acaaaga
//