LOCUS EY193444 577 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa94d04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193444 VERSION EY193444.1 GI:159496322 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 577) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 844 was extended to value 845 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 847 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..577 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc agcgcatttc ctcggccttc tccctggtcc agctgttcat tccgggccca 61 gtcagcattc tctacggcga agagttgggg ctgctcagcg gaagtgcctc ggccagcaaa 121 aagtcgaatc gggtcgtgta cccgtggaac aacggaactg gcctgggctt ctcggaggga 181 aaactgcaga cctacttctc cattccgagc accatttcca atggcaactt tgagagccaa 241 aagggggatc cgcactcgcc actcaatgtg ttcaagacgg ttgccaaact ccgccagaac 301 cccgacttca ttttgggcga gttcgagcac acgaagccgg ccgacattga tctgtacgtg 361 gtgaagcgct acccagccgg cagcggcttc cacgtgctgg ccgtgaactg gaacggagag 421 cagcgctcgc tgagcaacgg aaaattgccc gtggagctga aggaggcgaa actgtcttcg 481 gtggaagtgc tcgcctctgc cggggagccg aacgccccgt ttggggaagg tggacctgag 541 cgagggcgcc aacccactag atgtgagcgc atacgga //