LOCUS EY193441 231 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa94d01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193441 VERSION EY193441.1 GI:159496290 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 231) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 819 was extended to value 819 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 426 PolyA tail found inside read...SR being truncated to 382 Potential full insert read...vec-to-vec length: 303 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..231 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca cttcagctcc agcggggacg gctactgcaa cctggtgaag aacaacatct 61 ggtgccaggc agtcgcactg agcgcctgag ctggaagacg caaagggaaa gggagaaaga 121 aggaagagaa gagaccccgg cagttccact tcgccccaaa tacccggcac agtgcaataa 181 ccactcaaaa gagacatcaa atattttgct tagaatagag agaacggcac a //