LOCUS EY193439 245 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa94c11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193439 VERSION EY193439.1 GI:159496288 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 245) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 808 was extended to value 809 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 441 PolyA tail found inside read...SR being truncated to 397 Potential full insert read...vec-to-vec length: 317 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..245 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat tcggccaaat gctcactcac tctcttttcg tcttttctcc gctgttctgt 61 cctttcctct ttctccgccc aagtgatatc atccactcca gatcacaaca atttctccaa 121 actctttcga ttgcgggaac accaaccaaa tggaaaaata cttaaactct tttgattctt 181 tgccccaaag tggaacacat ttgtttacca ttcttgcgat tcggataaat gagaatttag 241 aatga //