LOCUS       EY193427                 505 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94b08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pdb|1UAY|A Chain A, Crystal Structure Of Type Ii
            3-Hydroxyacyl-Coa Dehydrogenase From Thermus Thermophilus Hb8
            pdb|1UAY|B Chain B, Crystal Structure Of Type Ii 3-Hydroxyacyl-Coa
            Dehydrogenase From Thermus Thermophilus Hb8, mRNA sequence.
ACCESSION   EY193427
VERSION     EY193427.1  GI:159496250
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 830 was extended to value 831 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 699
            PolyA tail found inside read...SR being truncated to 656
            Potential full insert read...vec-to-vec length: 577
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac aacgccaaca gcaacatccg ggtggtggcc attgcgcccg ggctgatgaa
       61 cacgccgctg atgaactcgc tgccggagaa ggccaagaag ttcctcgcct ccactgtgcc
      121 ttttccgccg cgccttggcg agccggacga cttcgcggcg ctggtggagc acattctgga
      181 gaacccgtac atcaacggga ccgtgatacg attggatggc gctcttcgca tgccctgaac
      241 ggtggctgct gctgctgctg aagacactcg atcattcatt cttcacacac actctgggcc
      301 tttgccgcca gtattgctca cttccaatcg acgcttcttt ttattagaca tttttattta
      361 ttttccatcc catatgccga aaggccaatt tttgggacat ttatgcaaat ttctggacgg
      421 aatggatgcg tcagtggaac aatcgatggc aaaaagttgt cctccaaata ttccacattg
      481 tttggttaaa cgtagcattt gttga
//