LOCUS       EY193423                 315 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193423
VERSION     EY193423.1  GI:159496215
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 818 was extended to value 818 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 509
            PolyA tail found inside read...SR being truncated to 466
            Potential full insert read...vec-to-vec length: 387
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcgg ggcaaacgca attcgcatgg accagtttgt tgcgtcgcca tgatcaggct
       61 gtggttgtac acgtttcggc cttcaattag tagtctcagt ttgatccttt tcttcgcaaa
      121 ctctcatccc gcgcttcttt ccctccgttc tcttttcctc attacttcgg tttcatgccc
      181 atttcattgc acctgtagta ataacacttt caacctgtct ctactgtctt ttctgtacca
      241 tgttttgtca cctgcaaata taccaaacag aattctgata aaattacgcg cattcaaaaa
      301 aaaaaaaaaa aaaca
//