LOCUS       EY193421                 363 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193421
VERSION     EY193421.1  GI:159496213
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 557
            PolyA tail found inside read...SR being truncated to 515
            Potential full insert read...vec-to-vec length: 435
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa acagatccga gaaatgctgt gcctctttaa cgacatgatc tggaaagaaa
       61 agacggacgt ttgtgctgaa tacagaatac acatgccaga cgaggaattg ccattgccac
      121 tctcagagca accgagcacc agcggaggcg gaaaccatta ggcgaatgag ttttttttgc
      181 agcattttag acatttcaat ttgtttggct taggaaatgc atccacattt gacgctacag
      241 ctttatgttt ttgactaatc cctttattta caggacaact taagcaattt cactatctgt
      301 tttcaggaca tctaatcact ttcactctct tgtggaataa gggaatatga tttgttttcc
      361 aga
//