LOCUS       EY193420                 330 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa94a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193420
VERSION     EY193420.1  GI:159496212
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 718 was extended to value 718 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 523
            PolyA tail found inside read...SR being truncated to 481
            Potential full insert read...vec-to-vec length: 402
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaac gagcgcacga ccaaaaacct ggacgtgttc aagcagcgcc tggaggtgct
       61 gcacaccaac ctgaggtgga tggagcgcaa cttcgcaccg cttgccgagt ggttccggga
      121 gcacagtccc gccacggaca acggggagga agagccgagc cacaacggct ttgcgggggc
      181 gccgatcgcg tgaggacagt gctcccccca atcccagctc aatgtgtgat atcatttcca
      241 cttcctttct aaactctaat ttttcaaatt taccaatttt ttgtctgtat gtgttttttc
      301 ggaactttat tttagaacat tacaaatata
//