LOCUS EY193406 283 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa93h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193406 VERSION EY193406.1 GI:159496170 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 283) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 810 was extended to value 810 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 491 PolyA tail found inside read...SR being truncated to 435 Potential full insert read...vec-to-vec length: 355 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..283 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt cgtaacttcg ggaaaagagt gcggaaggta aggacgggat agccgaagcg 61 aagaagggag aaaaaacgga aggtgaagtt gacgttgaac aagacggaaa aagtgcagaa 121 gaagagaaag cggctgagga gcagaatgaa taaggcgctg aagatgatcg acatggggac 181 gactttttcg ggatgtgatt cattcacttt ggaccttgtt gttcttgacg agttcttcgt 241 tctgttgttt cccatatttt aatcaaataa aaattttaaa tga //