LOCUS       EY193395                 309 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93g02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193395
VERSION     EY193395.1  GI:159496141
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 764 was extended to value 764 (,)
            putative full insert read: vector-to-vector length is 415 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 567
            PolyA tail found inside read...SR being truncated to 461
            Potential full insert read...vec-to-vec length: 452
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg ccatcgacgg aggagtgttc ttcgaggacg gaacccgacg tcgctgagtg
       61 gcagaagaga gtcgtgtcca actcgaggtc tttcaactac attcgtattt caacacttcg
      121 gacaaaactt caagcggaga gaaaccagtt cgcatttatc tgatttcaaa aaatgaagaa
      181 gagaataaat aaagacaaac gaagatgcat ctacattcct ggtacatcca cagaagggac
      241 aaaagactcg aaattgcatg tgatttcggt gtttgttctt tttcacgtag agtgcaaagt
      301 ttaatgtga
//