LOCUS       EY193374                 254 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa93e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193374
VERSION     EY193374.1  GI:159496120
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 851 was extended to value 851 (,)
            putative full insert read: vector-to-vector length is 331 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 479
            PolyA tail found inside read...SR being truncated to 405
            Potential full insert read...vec-to-vec length: 326
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gtaactgacg cggatctatg atggaccggc ccaaatttgt tttgcattat
       61 tcgcacttag aatcgcattt tgacatatca aaaccaccca tacacacccc atccatattt
      121 atttgagtgc ataattttta attttcctgt tatcctccaa ctgcataata gacacacacc
      181 caaagccatg ttgatttcac ccaagatatt tattttagat ggtactgaca atttatgtat
      241 aaatattttg ttta
//